There are many published methods for MU determination (i.e. calculation of the standard uncertainty) however; there is no “one size fits all” approach that is able to be applied to laboratory pathology. As such the methods available below will be selected on an assay by assay basis.
- The Coefficient of Variation (CV)
- Single Laboratory Validation approach (using QC data + EQA)
- Alternative Single Laboratory Validation approach (QC data + certified reference values)
- Top-Down Approach (QC data)
- Guide to the Uncertainty of Measurement (GUM)
- Alternative GUM approach (Type A and Type B data Root Sum of Squares method)
- Inter laboratory comparison (Proficiency testing/EQA)
- Monte Carlo Simulation
- Inter Observer Agreement (Inter Rater Reliability)
Pathology Uncertainty 