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What can a histogram tell me about my laboratory data?

A histogram shows how often results fall into successive ranges. It can reveal where most observations sit, how far they spread and whether there are gaps, long tails or clusters worth investigating.

Worked example at a glance

48 results

Receipt-to-authorisation times

5 minutes

Width of each interval

2 workflows

24 results from each

Read the axes before interpreting the shape

Each bar counts observations in a range of values, called a bin. In this guide, every bin is five minutes wide, so taller bars contain more results. Adjacent bins represent adjacent ranges on a numerical scale. [1]

Start by looking at where the results are concentrated, then look for gaps, tails and clusters. Bin width and bin boundaries affect how the same data look. Very wide bins can hide features, while very narrow bins can make a modest sample look fragmented. If a feature matters, see whether it remains visible with another sensible choice of bins. [1]

See what changes when you label the workflows

A fictional laboratory records 48 receipt-to-authorisation times for one test. In the pooled histogram, results gather in two separate bands.

Two histograms with matching axes show 48 turnaround times. The upper plot shows two clusters in the pooled data. The lower plot shows the shorter times belong to workflow A and the longer times to workflow B in this example.
Fictional turnaround times. Both panels use five-minute intervals and the same count scale. Open the figure full size.

The gap prompts a question about whether the times came from different processes. When the laboratory labels each observation by workflow, the shorter times belong to workflow A and the longer times to workflow B in this set.

The split gives a plausible explanation for the two clusters. To understand the difference, the laboratory would still need information about how the cases were routed, how the timestamps were captured and what else varied between workflows.

Use the shape to choose the next view

The overall range in the table summarises the shortest and longest observed times. The histogram shows that the times did not fill that range evenly.

ObservationsCountObserved range
All results4824–68 min
Workflow A2424–39 min
Workflow B2453–68 min
The observed range of the pooled data spans a gap with no recorded times.

A histogram rearranges the observations by value. It loses their time order. To see whether turnaround changed after an event or varied by day, plot the original times in chronological order.

When comparing separate histograms, use matching bin boundaries and axis scales. If groups are very different in size, proportions can make their shapes easier to compare. Show the sample sizes as well.

Describe what the graph showed

State which observations were included and which bin width you used. Separate the feature in the histogram from the explanation you plan to check.

Example wordingThe 48 recorded times formed two clusters using five-minute bins. In these observations, the shorter cluster belonged to workflow A and the longer cluster to workflow B.

Member Learning

Plan a laboratory study and prepare your data

Work through a laboratory study, from planning and checking the source data to summarising results and explaining their limits.

Sources and related reading

Read when to use SD, CV, standard error or a confidence interval

By Stephen MacDonald · Suggest a correction · Back to Find a guide